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M94A0335.TXT
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1994-10-08
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Document 0335
DOCN M94A0335
TI Phosphorescence and optically detected magnetic resonance investigation
of the binding of the nucleocapsid protein of the human immunodeficiency
virus type 1 and related peptides to RNA.
DT 9412
AU Lam WC; Maki AH; Casas-Finet JR; Erickson JW; Kane BP; Sowder RC 2nd;
Henderson LE; Department of Chemistry, University of California, Davis
95616.
SO Biochemistry. 1994 Sep 6;33(35):10693-700. Unique Identifier : AIDSLINE
MED/94355292
AB The RNA and DNA complexes of nucleocapsid protein p7.Zn (NCp7.Zn) of the
human immunodeficiency virus type 1 (HIV-1) are studied by
phosphorescence and optically detected magnetic resonance (ODMR). The
single tryptophan, Trp37, which is located on the C-terminal zinc finger
domain is used as an intrinsic probe. Reductions in the triplet state
zero-field splitting (zfs) D parameter of Trp37 upon complex formation
with poly(I) and poly(U) are observed. These results, in conjunction
with the phosphorescence red-shifts and triplet state lifetime
reductions that are observed, suggest the presence of aromatic stacking
interactions between NCp7.Zn and the bases of the RNA polymers. An
alteration of the intersystem crossing pattern upon complex formation,
in addition to the above mentioned spectroscopic shifts, also is
consistent with previously observed tryptophans that undergo stacking
interactions with DNA bases [Zang, L.-H., Maki, A.H., Murphy, J.B., &
Chase, J.W. (1987) Biophys. J. 52, 867-872. Tsao, D.H.H., Casas-Finet,
J.R., Maki, A.H., & Chase, J.W. (1989) Biophys. J. 55, 927-936]. These
conclusions support those from a recent ODMR study [Lam, W.-C., Maki,
A.H., Casas-Finet, J.R., Erickson, J.W., Sowder, R.C., II, & Henderson,
L.E. (1993) FEBS Lett. 328, 45-48] of NCp7.Zn binding to 5-mercurated
polyuridylic acid [poly(5-HgU)] in which stacking interactions between
the RNA and NCp7.Zn are inferred from the observation of an external
heavy atom effect induced on Trp37.(ABSTRACT TRUNCATED AT 250 WORDS)
DE Amino Acid Sequence Capsid/*METABOLISM Gene Products, gag/*METABOLISM
HIV-1/*CHEMISTRY Luminescence Molecular Sequence Data RNA-Binding
Proteins/*CHEMISTRY RNA, Viral/*METABOLISM Spectrum Analysis Support,
U.S. Gov't, P.H.S. *Zinc Fingers JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).